Highway 7 is a provincially maintained highway in the province of Ontario, Canada. At its peak, Highway 7 measured a total distance of 716 km in length, stretching from Highway 40 east of Sarnia in southwestern Ontario to Highway 417 southwest of Kanata in eastern Ontario. Ontarians often referred to Highway 7 as the Trans-Canada Highway although it only forms part of the Trans-Canada Highway east of Highway 12 near Sunderland.

In 1997 and 1998, the Ontario Ministry of Transportation downloaded portions of the highway to regional and municipal governments. Because of this, there are now two (once three) separated sections of Highway 7.