New York State Route 27 (abbreviated NY 27) is a 122.28 mile long state highway extending from Interstate 278 in the New York City borough of Brooklyn to Montauk Point State Park on Long Island, New York, United States. Its two most prominent components are Sunrise Highway and Montauk Highway.

East of the interchange with the Heckscher State Parkway in Islip Terrace, NY 27 acts as the primary east-west highway on southern Long Island.

The entire route in Suffolk, Nassau, and Queens was designated by the New York State Senate as the POW/MIA Memorial Highway.

On the South Shore, nearly every town is accessible through Sunrise Highway.