The Napa Valley Wine Train is operated by the Napa Valley Railroad . The train is a privately operated excursion train that runs between Napa and St. Helena, California. Much of the rail line parallels State Route 29 after leaving the City of Napa and passes the towns of Yountville, Rutherford and Oakville. The route skirts many of the region's notable vineyards and wineries located in the picturesque Napa Valley.