Main Street, U.S.A. is the first "themed land" inside the entrance of the many Magic Kingdom-style parks run by The Walt Disney Company around the world.

Each Main Street, U.S.A. (except Tokyo Disneyland) has a train station above the entrance. At Disneyland, Disneyland Paris and Hong Kong Disneyland, Sleeping Beauty Castle stands in the distance beyond the end of the street. At the Magic Kingdom and Tokyo Disneyland, Cinderella Castle stands at the end.

Main Street, U.S.A. is home to City Hall, in which the Guest Relations office is located. Further along Main Street, the names painted in the windows on Main Street serve as credits for some of the many people, Imagineers and others, who contributed in some way to the creation of Disneyland. Largely they appear as fictional businesses (gyms, realtors, dentists and the like), and they often refer to a hobby or interest that the person in question had. Ub Iwerks's window, for example, refers to his prowess with cameras. For Disneyland's 50th anniversary, on July 17, 2005, a first-story window on each Main Street was unveiled with a dedication to all the cast members (employees) who had worked for Disney throughout the years.