MEDLINE (Medical Literature Analysis and Retrieval System Online) is a literature database of life sciences and biomedical information. It includes medicine, nursing, pharmacy, dentistry, veterinary medicine, and health care. MEDLINE covers much of the literature in biology and biochemistry, and fields such as molecular evolution. Listing of an article or journal in MEDLINE is not endorsement.

Compiled by the U.S. National Library of Medicine (NLM), MEDLINE is freely available on the Internet and searchable via PubMed and NLM's National Center for Biotechnology Information's Entrez system.