Lady Eleanor Brandon (1519 - September 27, 1547) was the third child and second daughter of Charles Brandon, 1st Duke of Suffolk and Mary Tudor, the former Queen consort of France. She was a younger sister of Henry Brandon, 1st Earl of Lincoln and Lady Frances Brandon, and an elder half-sister of Henry Brandon, 2nd Duke of Suffolk and Charles Brandon, 3rd Duke of Suffolk.

Her paternal grandparents were Sir William Brandon and Elizabeth Bruyn. Her maternal grandparents were Henry VII of England and his queen consort Elizabeth of York. She was thus a niece of Henry VIII.