The benthic zone is the ecological region at the lowest level of a body of water such as an ocean or a lake, including the sediment surface and some sub-surface layers. Organisms living in this zone are called benthos. They generally live in close relationship with the substrate bottom; many such organisms are permanently attached to the bottom. Generally, these include life forms that tolerate cool temperatures and low oxygen levels, but this depends on the depth of the water. For information on animals that live in the deepest areas of the oceans see aphotic zone. The superficial layer of the soil lining the given body of water is an integral part of the benthic zone, as it influences greatly the biological activity which takes place there. Examples of contact soil layers include sand bottoms, rock outcrops, coral, and bay mud.

For comparison, the pelagic zone is the descriptive term for the ecological region above the benthos, including the water-column up to the surface. Depending on the water-body, the benthic zone may include areas which are only a few inches below water, such as a stream or shallow pond; at the other end of the spectrum, benthos of the deep ocean includes the bottom levels of the oceanic abyssal zone.