Barstow is a city in San Bernardino County, California, United States. The population was 21,119 at the 2000 census.

Barstow is a major regional transportation center. Several major highways including Interstate 15 and Interstate 40 converge in the city. It is the site of a large rail classification yard, belonging to the BNSF Railway. The Union Pacific Railroad also runs through town on trackage rights on BNSF's main line to Daggett 10 miles east, where it heads to Salt Lake City, and the BNSF heads to Chicago.

Barstow is home to Marine Corps Logistics Base Barstow and is the closest city to Fort Irwin Military Reservation.

Barstow is disproportinately represented in popular culture, due to its small population, remoteness and relative isolation. Route 66 runs through Barstow and the town's name is mentioned in the lyrics to the song, "Route 66" and in the Black Eyed Peas song "Like That" as Will.I.Am raps that he "(has) a style that's way out like Barstow" commenting on Barstow's distance from the BEP's home city of Los Angeles. Barstow is also mentioned in Hunter S. Thompson's novel Fear and Loathing in Las Vegas, in Quentin Tarantino's film Kill Bill Vol. II, and in numerous other films and television shows.

Johnny Carson of the Tonight Show uses Barstow as a target in many of his jokes. Spike Jones uses Barstow in his song "Barstool Cowboy from Old Barstow." A more serious composition by Harry Partch is entititled "Barstow: Eight Hitchhiker Inscriptions from a Highway Railing at Barstow" and is based upon his travels through the Mojave desert town in the early 1940s.

Sheryl Crow also makes reference to Barstow, CA in her song Leaving Las Vegas... "Used to be I could drive up to Barstow for the night Find some crossroad trucker To demonstrate his might But these days it seems Nowhere is far enough away So Im leaving Las Vegas today"